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材料工程  2020, Vol. 48 Issue (5): 83-90    DOI: 10.11868/j.issn.1001-4381.2019.001004
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
可注射乙酰化乙二醇壳聚糖/泊洛沙姆复合水凝胶的制备及药物缓释研究
李进1, 候冰娜1, 韩超越1, 倪凯1, 赵梓年1, 李征征1,2,3,4
1. 天津科技大学 化工与材料学院, 天津 300457;
2. 天津科技大学 天津市制浆造纸重点实验室, 天津 300457;
3. 复旦大学 聚合物分子工程国家重点实验室, 上海 200433;
4. 天津科技大学 天津市海洋资源与化学重点实验室, 天津 300457
Preparation of injectable N-acetyl glycol chitosan/poloxamer composite hydrogel for drug release
LI Jin1, HOU Bing-na1, HAN Chao-yue1, NI Kai1, ZHAO Zi-nian1, LI Zheng-zheng1,2,3,4
1. College of Chemical Engineering and Materials, Tianjin University of Science & Technology, Tianjin 300457, China;
2. Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science & Technology, Tianjin 300457, China;
3. State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China;
4. Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science & Technology, Tianjin 300457, China
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摘要 泊洛沙姆(poloxamer)是一种温敏性聚合物,在浓度为15.0%(质量分数,下同)~30.0%时可形成凝胶。为改善泊洛沙姆在体温下的成胶浓度和药物缓释性能,以泊洛沙姆407为基底,与新型温敏性乙酰化乙二醇壳聚糖复合,制得了温敏性乙酰化乙二醇壳聚糖/泊洛沙姆复合水凝胶。通过傅里叶变换红外光谱(FT-IR)、试管倒置法、旋转流变仪、扫描电子显微镜(SEM)和紫外-可见分光光度计(UV-vis)对乙酰化乙二醇壳聚糖/泊洛沙姆的结构、温敏性、力学性能、微观形貌和体外药物释放性能进行表征。结果表明,乙酰化乙二醇壳聚糖/泊洛沙姆溶液具有热可逆温敏性溶胶-凝胶转变行为。通过控制乙酰化乙二醇壳聚糖/泊洛沙姆的质量比,能够使溶胶-凝胶转变温度处于室温与体温(25~37℃)之间,缩短凝胶化时间(382 s),降低泊洛沙姆407在体温下的成胶浓度(6%)。乙酰化乙二醇壳聚糖/泊洛沙姆复合水凝胶具有高度孔隙化的三维结构,其孔径大小处于10~60 μm范围内,且表现出较高的力学性能。乙酰化乙二醇壳聚糖/泊洛沙姆复合水凝胶对抗癌药物吉西他滨具有缓释作用,载药凝胶的释药时间可达72 h。乙酰化乙二醇壳聚糖/泊洛沙姆复合水凝胶在可注射药物缓释载体方面具有重要的应用前景。
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李进
候冰娜
韩超越
倪凯
赵梓年
李征征
关键词 泊洛沙姆乙酰化乙二醇壳聚糖溶胶-凝胶转变药物缓释载体    
Abstract:Poloxamer is a thermo-sensitive polymer that can form gel at a concentration of 15.0%(mass fraction, the same as below)-30.0%.In order to decrease the gelatinization concentration and improve drug release properties of poloxamer at body temperature, thermo-sensitive N-acetyl glycol chitosan/poloxamer composite hydrogel was prepared by complexing N-acetyl glycol chitosan with poloxamer 407 (GC/P407).The structure, thermo-sensitivity, mechanical properties, morphology and in vitro drug release properties of GC/P407 were characterized by FT-IR, tube inverting method, rheometer, SEM and UV-vis spectroscopy. The GC/P407 solution shows reversible thermo-sensitive sol-gel transition behavior, and the sol-gel transition temperature is well controlled in the range of 25-37℃ by regulating the ratio of GC/P407, which shortens the gelation time and the gelatinization concentration(6%) of poloxamer 407 at body temperature. GC/P407 composite hydrogel, which has a highly porous three-dimensional structure with pore size of 10-60 μm as demonstrated by SEM, exhibits high mechanical properties. In addition, the GC/P407 composite hydrogel shows sustained release behavior of the anticancer drug gemcitabine, and the release time of the drug-loaded gel can reach 72 h. GC/P407 composite hydrogel shows the potential for biomedical application as injectable drug delivery carrier.
Key wordspoloxamer    N-acetyl glycol chitosan    sol-gel transition    drug release carrier
收稿日期: 2019-11-05      出版日期: 2020-05-28
中图分类号:  O648.17  
通讯作者: 李征征(1984-),女,副研究员,博士,主要从事智能高分子水凝胶及其生物医用的研究,联系地址:天津市滨海新区经济技术开发区第十三大街29号天津科技大学泰达校区化工与材料学院(300457),E-mail:li.z.z@tust.edu.cn     E-mail: li.z.z@tust.edu.cn
引用本文:   
李进, 候冰娜, 韩超越, 倪凯, 赵梓年, 李征征. 可注射乙酰化乙二醇壳聚糖/泊洛沙姆复合水凝胶的制备及药物缓释研究[J]. 材料工程, 2020, 48(5): 83-90.
LI Jin, HOU Bing-na, HAN Chao-yue, NI Kai, ZHAO Zi-nian, LI Zheng-zheng. Preparation of injectable N-acetyl glycol chitosan/poloxamer composite hydrogel for drug release. Journal of Materials Engineering, 2020, 48(5): 83-90.
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http://jme.biam.ac.cn/CN/10.11868/j.issn.1001-4381.2019.001004      或      http://jme.biam.ac.cn/CN/Y2020/V48/I5/83
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