Preparation of injectable N-acetyl glycol chitosan/poloxamer composite hydrogel for drug release
LI Jin1, HOU Bing-na1, HAN Chao-yue1, NI Kai1, ZHAO Zi-nian1, LI Zheng-zheng1,2,3,4
1. College of Chemical Engineering and Materials, Tianjin University of Science & Technology, Tianjin 300457, China;
2. Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science & Technology, Tianjin 300457, China;
3. State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China;
4. Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science & Technology, Tianjin 300457, China
Abstract：Poloxamer is a thermo-sensitive polymer that can form gel at a concentration of 15.0%(mass fraction, the same as below)-30.0%.In order to decrease the gelatinization concentration and improve drug release properties of poloxamer at body temperature, thermo-sensitive N-acetyl glycol chitosan/poloxamer composite hydrogel was prepared by complexing N-acetyl glycol chitosan with poloxamer 407 (GC/P407).The structure, thermo-sensitivity, mechanical properties, morphology and in vitro drug release properties of GC/P407 were characterized by FT-IR, tube inverting method, rheometer, SEM and UV-vis spectroscopy. The GC/P407 solution shows reversible thermo-sensitive sol-gel transition behavior, and the sol-gel transition temperature is well controlled in the range of 25-37℃ by regulating the ratio of GC/P407, which shortens the gelation time and the gelatinization concentration(6%) of poloxamer 407 at body temperature. GC/P407 composite hydrogel, which has a highly porous three-dimensional structure with pore size of 10-60 μm as demonstrated by SEM, exhibits high mechanical properties. In addition, the GC/P407 composite hydrogel shows sustained release behavior of the anticancer drug gemcitabine, and the release time of the drug-loaded gel can reach 72 h. GC/P407 composite hydrogel shows the potential for biomedical application as injectable drug delivery carrier.
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