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材料工程  2008, Vol. 0 Issue (10): 106-109    
  "2008第七届中国国际纳米科技(武汉)研讨会"论文专辑 本期目录 | 过刊浏览 | 高级检索 |
纳米银对大肠杆菌的抗菌作用及其机制
谢小保, 李文茹, 曾海燕, 欧阳友生, 陈仪本
1. 广东省微生物研究所, 广州, 510070;
2. 广东省菌种保藏与应用重点实验室, 广州, 510070
Study of Antimicrobial Activity and Mechanism of Silver Nanoparticles on Escherichia coli
XIE Xiao-bao, LI Wen-ru, ZENG Hai-yan, OUYANG You-sheng, CHEN Yi-ben,
1. Guangdong Institute of Microbiology, Guangzhou 510070, China;
2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, China
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摘要 以大肠杆菌为研究对象,对纳米银的抗菌效果进行了研究,并对其抗菌机制做了初步探讨。纳米银对大肠杆菌的抑制生长曲线的结果表明,20μg/mL的纳米银能够完全抑制106cfu/mL的大肠杆菌细胞生长,纳米银使大肠杆菌的延滞期加长,并且纳米银浓度越高,延滞期越长。采用透射电镜观察了经纳米银粒子处理过的大肠杆菌细胞形态变化过程,结果显示纳米银粒子先在细胞壁上产生小的孔洞,通过这些孔洞进入周质空间,导致细胞膜成分渗漏和破坏细胞膜,进而进入细胞内部。进入细胞内部的纳米银粒子使DNA浓缩呈紧张态,并与破损细菌的细胞质结合积聚,最后引起胞内物质流失。另外,纳米银对大肠杆菌总DNA影响的分析表明,随着纳米银浓度的增高,大肠杆菌总DNA样品降解的程度增大。
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谢小保
李文茹
曾海燕
欧阳友生
陈仪本
关键词 纳米银大肠杆菌抗菌作用    
Abstract:The antimicrobial effect of the nanosilver on Escherichia coli and the antimicrobial mechanism was studied elementarily.Experimental results indicated that the silver nanoparticles of 20μg/mL could inhibit completely the growth of 106cfu/mL cells in liquid LB medium.The growth curves showed that silver nanoparticles prolonged the lag phase of E.coli,and the higher of the concentration of silver nanoparticles,the longer of the lag phase of E.coli.Transmission electron microscopy(TEM) was used to evaluate the cell morphology of both the normal and the treated E.coli.The observation with TEM suggested that silver nanoparticles lead to the formation of "pits" in cell wall of the bacteria,and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane.Then the silver nanoparticles could enter into the bacterial cell,which not only condensed DNA,but also combined and coagulated with the cytoplasm of damaged bacteria.Finally,silver nanoparticles resulted in the leakage of cytoplasmic component.Moreover,the analysis of agar gel electrophoresis demonstrated that silver nanoparticles could increase the decomposability of genome DNA.
Key wordsnanosilver    Escherichia coli    antimicrobial activity
收稿日期: 2008-06-11      出版日期: 2008-10-20
1:  R318.08  
基金资助:广东省自然科学基金资助项目(A06104481)
作者简介: 谢小保(1966- ), 男, 硕士, 副研究员, 主要从事抗菌材料的抗菌作用机理及微生物耐药机理研究, 联系地址: 广州市先烈中路100号大院广东省微生物研究所(510070).E-mail: xxJaobao@tom.com
引用本文:   
谢小保, 李文茹, 曾海燕, 欧阳友生, 陈仪本. 纳米银对大肠杆菌的抗菌作用及其机制[J]. 材料工程, 2008, 0(10): 106-109.
XIE Xiao-bao, LI Wen-ru, ZENG Hai-yan, OUYANG You-sheng, CHEN Yi-ben,. Study of Antimicrobial Activity and Mechanism of Silver Nanoparticles on Escherichia coli. Journal of Materials Engineering, 2008, 0(10): 106-109.
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http://jme.biam.ac.cn/CN/      或      http://jme.biam.ac.cn/CN/Y2008/V0/I10/106
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