Montmorillonite(MMT) was utilized to absorb three types of cationic photosensitizers, namely, meso-tetra(4-N-methylpyridyl)porphyrine tetratosylate (TMPyP), Zn(Ⅱ) meso-tetra (N-methyl-4-pyridyl) porphine tetrachloride (Zn-TMPyP) and methylene blue (MB), the as-obtained mixtures were then evenly dispersed in aqueous polyvinyl alcohol-styrylpyridine salt condensate (PVA-SbQ) solution, corresponding photoactive MMT/PS hydrogels were prepared by the assay of UV cross-linking eventually. Transmission electron microscopy (TEM) was employed to analyse morphology of MMT and one as-prepared MMT/PS hydrogel. Scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) were applied to analyse the internal morphology and thermal properties of the as-prepared hydrogels. Finally, swelling properties and antibacterial effect against staphylococcus aureus (S. aureus) were investigated. The results show that three photoactive MMT/PS hydrogels are successfully prepared with good swelling properties. The as-prepared hydrogels exhibit inactivation against S. aureus to some extent, furthermore, the hydrogel which immobilizes Zn-TMPyP reveals best inactivation efficacy, with a sterilization rate of 98.49%.
LYU S H , LI Y , YANG W Q , et al. Research progress on preparation and application of graphene oxide/chitosan biocomposites[J]. Journal of Materials Engineering, 2016, 44 (10): 119- 128.
SPAGNUL C , TURNER L C , BOYLE R W . Immobilized photosensitizers for antimicrobial applications[J]. J Photochem Photobiol B-Biol, 2015, 150, 11- 30.
SCHLACHET I , SOSNIK A . Protoporphyrin IX-modified chitosan-g-oligo (NiPAAm) polymeric micelles:from physical stabilization to permeability characterization in vitro[J]. Biomater Sci, 2017, 5 (1): 128- 140.
ALVES E , FAUSTINO M A F , NEVES M , et al. Potential applications of porphyrins in photodynamic inactivation beyond the medical scope[J]. J Photochem Photobiol C-Photochem Rev, 2015, 22, 34- 57.
CAVALLARO A , TAHERI S , VASILEV K . Responsive and "smart" antibacterial surfaces:common approaches and new developments (review)[J]. Biointerphases, 2014, 9 (2): 10- 19.
BUJDAK J , KOMADEL P . Interaction of methylene blue with reduced charge montmorillonite[J]. J Phys Chem B, 1997, 101 (44): 9065- 9068.
BETTANIN F , FONTINELLES T A D , MACIEL C D , et al. Aggregation of photosensitizers:the role of dispersion and solvation on dimer formation energetics[J]. Theoretical Chemistry Accounts, 2015, 134 (12): 11- 23.
ZHANG H F , HUSSAIN I , BRUST M , et al. Aligned two-and three-dimensional structures by directional freezing of polymers and nanoparticles[J]. Nat Mater, 2005, 4 (10): 787- 793.
WU J , MEREDITH J C . Assembly of chitin nanofibers into porous biomimetic structures via freeze drying[J]. ACS Macro Lett, 2014, 3 (2): 185- 190.
CUI J , WANG Q , WEI Q . Synthesis and characterization of cross-linked SbQ/montmorillonite composites[J]. Polymer Materials Science & Engineering, 2015, 31 (7): 159- 162.
ROTOMSKIS R , STRECKYTE G , BAGDONAS S . Phototransformations of sensitizers 1. significance of the nature of the sensitizer in the photobleaching process and photoproduct formation in aqueous solution[J]. J Photochem Photobiol B-Biol, 1997, 39, 167- 171.
HORVATH O , HUSZANK R , VALICSEK Z , et al. Photophysics and photochemistry of kinetically labile, water-soluble porphyrin complexes[J]. Coordination Chemistry Reviews, 2006, 250 (13/14): 1792- 1803.
XU F L , LI Y B , LI J D , et al. Swelling properties of nano-hydroxyaptite/polyvinyl alcohol hydrogel composite[J]. Journal of Materials Engineering, 2005, (7): 15- 18.
CUI X T , LIU J L , ZHANG D K , et al. Sliding friction behavior of PVA/HA composite hydrogels under different contacting scales[J]. Journal of Materials Engineering, 2016, 44 (5): 59- 64.
GOMEZ I , OTAZO E M , HERNANDEZ H , et al. Thermal degradation study of PVA derivative with pendant phenylthionecarbamate groups by DSC/TGA and GC/MS[J]. Polym Degrad Stabil, 2015, 112, 132- 136.
XU J , BAI H Y , WANG M , et al. Properties of hyaluronan/PVA-SbQ composite films processed by casting[J]. Polymers & Polymer Composites, 2013, 21 (1): 55- 60.
REDMOND R W , GAMLIN J N . A compilation of singlet oxygen yields from biologically relevant molecules[J]. Photochem Photobiol, 1999, 70 (4): 391- 475.
ZHAO Z , LI Y , MENG S , et al. Susceptibility of methicillin-resistant staphylococcus aureus to photodynamic antimicrobial chemotherapy with α-d-galactopyranosyl zinc phthalocyanines:in vitro study[J]. Lasers in Medical Science, 2014, 29 (3): 1131- 1138.