γ辐射和EDC/NHS改性对胶原壳聚糖支架性能的影响

doi:10.11868/j.issn.1001-4381.2018.001498

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摘要

以5%梯度制备11组壳聚糖质量分数为0%~50%的胶原壳聚糖支架。使用γ辐射和EDC/NHS分别改性处理各组胶原壳聚糖支架，采用傅里叶变换红外光谱仪（FTIR）和扫描电镜（SEM）分析支架内部结构，利用吸水率、孔隙率、降解率和力学性能等指标对其性能进行检测，研究γ辐射和EDC/NHS改性对胶原壳聚糖支架性能的影响。结果表明：γ辐射和EDC/NHS改性均能使胶原与壳聚糖产生交联，壳聚糖的加入改善了γ辐射对支架分子结构的损伤；EDC/NHS改性支架的微结构好于γ辐射支架；两种改性支架壳聚糖较优，质量分数均为25%；γ辐射和EDC/NHS改性均能使支架产生取向结构。

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	杜歌
	魏莉
	刘自双
	武继民
	陈子浩
	田丰

关键词 ：胶原壳聚糖支架, γ辐射, EDC/NHS, 交联, 改性

Abstract：

With chitosan mass fraction changing from 0% to 50% by 5%, relative to collagen mass, the collagen-chitosan scaffolds were crosslinked by γ-irradiation and EDC/NHS. Fourier transform infrared spectroscopy (FTIR) and SEM were applied to analyze the structure. Water absorption rate, porosity, collagenase degradation and mechanical property were used to detect the properties. The effects of γ-irradiation and EDC/NHS modification on the properties of scaffolds were studied. The results show that both γ-irradiation and EDC/NHS modification induce crosslinking in collagen-chitosan scaffolds and chitosan greatly reduce the damage to molecular structure caused by γ-irradiation.The microstructure crosslinked by γ-irradiation is worse than those crosslinked by EDC/NHS. The optimal chitosan mass fraction is 25% for both γ-irradiation and EDC/NHS. γ-irradiation and EDC/NHS scaffold modification can lead to orientation structure.

Key words： collagen-chitosan scaffold γ-irradiation EDC/NHS crosslinking modification

收稿日期: 2018-12-27 出版日期: 2020-05-28

中图分类号:

R318.08

基金资助:天津市应用基础与前沿技术研究计划资助项目(13JCZDJC33400)

通讯作者: 陈子浩,田丰 E-mail: zjdyzxczh1111@126.com;tianfeng62037@163.com

作者简介: 田丰(1964-), 男, 研究员, 从事专业:急救材料与包装工程, 联系地址:北京市海淀区阜成路6号中国人民解放军总医院第六医学中心(100048), E-mail:tianfeng62037@163.com
陈子浩(1990-), 男, 助理研究员, 博士, 从事专业:生物医用材料, 联系地址:北京市海淀区阜成路6号中国人民解放军总医院第六医学中心(100048), E-mail:zjdyzxczh1111@126.com

引用本文:

杜歌, 魏莉, 刘自双, 武继民, 陈子浩, 田丰. γ辐射和EDC/NHS改性对胶原壳聚糖支架性能的影响[J]. 材料工程, 2020, 48(5): 106-111.
Ge DU, Li WEI, Zi-shuang LIU, Ji-min WU, Zi-hao CHEN, Feng TIAN. Effects of γ-irradiation and EDC/NHS modification on property of collagen-chitosan scaffolds. Journal of Materials Engineering, 2020, 48(5): 106-111.

链接本文:

http://jme.biam.ac.cn/CN/10.11868/j.issn.1001-4381.2018.001498 或 http://jme.biam.ac.cn/CN/Y2020/V48/I5/106

Fig.1 γ辐射支架结构分析
(a)壳聚糖质量分数50%时γ辐射组和对照组的FTIR光谱图；(b)壳聚糖与胶原可能的交联反应

Fig.2 Non组(1)，γ-ray组(2)和EDC组(3)不同壳聚糖质量分数时支架的SEM图
(a)0%;(b)25%;(c)50%

Table 1 3种壳聚糖质量分数下各组支架孔径

Fig.3 25%壳聚糖时3组支架的FTIR谱图

Fig.4 Non组，γ-ray组和EDC组在不同壳聚糖质量分数时的支架性能参数
(a)吸水率；(b)孔隙率；(c)横向弹性模量；(d)纵向弹性模量

Table 2 3组支架的吸水率、孔隙率、横向和纵向弹性模量

Fig.5 3种不同壳聚糖质量分数时Non组, γ-ray组和EDC组支架在Ⅰ型胶原酶作用不同时间的降解率

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