聚乳酸/聚乙二醇/羟基磷灰石多孔骨支架的3D打印制备及其生物相容性

doi:10.11868/j.issn.1001-4381.2020.000390

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摘要

聚乳酸（PLA）是一种应用广泛的生物高分子材料，但在应用过程中存在韧性、亲水性、生物活性差等缺点。用聚乙二醇（PEG）和羟基磷灰石（HA）对PLA进行改性。通过熔融共混制备不同质量比的PLA/PEG/HA复合3D打印线材，并通过分析PLA/PEG/HA线材的力学性能、结晶性能、热性能、流变性能等，筛选更适合熔融沉积成型（FDM）的3D打印成型线材，进而利用3D打印制备精度高的力学性能试样及生物相容性好、细胞可增殖和分化的生物多孔支架。结果表明：PEG的添加提高了PLA的韧性，降低了PLA的熔点。HA的添加则提高PLA/PEG/HA复合材料的弹性模量和冷结晶温度，同时HA也可以改善复合材料的加工性能。SEM与荧光标记结果表明多孔支架与细胞具有良好的生物相容性。生物支架对体外细胞的成功培养，为进一步发掘生物多孔支架在动物体内、生物医学及定制化应用方面提供了潜在可能。

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	范泽文
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	王艳
	郭静
	权慧欣
	徐兰娟

关键词 ：聚乳酸, 3D打印, 多孔支架, 生物相容性

Abstract：

Polylactic acid (PLA) is a widely used biopolymer material. However, there are disadvantages such as poor toughness, poor hydrophilicity, and poor biological activity in the application process. It was modified with polyethylene glycol (PEG) and hydroxyapatite (HA).3D printing filaments of PLA/PEG/HA with different mass ratios were prepared by melt blending. And by analyzing the mechanical properties, crystallization properties, thermal properties, rheological properties of PLA/PEG/HA filaments, the more suitable filaments for fused deposition modeling of 3D printing (FDM) were screened, and then the high precision mechanical samples and bioporous scaffolds with good biocompatibility, cell value-added and differentiation were 3D printed. The results show that the addition of PEG improves the toughness of PLA and lowers the melting point of PLA. The addition of HA increases the elastic modulus and cold crystallization temperature of PLA/PEG/HA composites, and HA can also improve the flowability of PLA/PEG/HA composites. SEM and fluorescent labeling results show that the porous scaffold has good biocompatibility. The successful cultivation of bioscaffolds in vitro cells provides potential for further exploration of bioporous scaffolds in animals, biomedicine, and customized applications.

Key words： polylactic acid 3D printing porous scaffold biocompatibility

收稿日期: 2020-04-30 出版日期: 2021-04-21

中图分类号:

TQ321.2

基金资助:国家自然科学基金(51503023);辽宁省自然科学基金重点项目(20170520305)

通讯作者: 王艳 E-mail: wyan@163.com

作者简介: 王艳(1983-), 女, 讲师, 博士, 从事半结晶聚合物及其复合材料加工过程中形态结构控制及性能、面向3D打印的高分子材料开发及其加工新方法等研究, 联系地址: 辽宁省大连市甘井子区轻工苑一号大连工业大学纺织与材料工程学院三楼305室(116034), E-mail: wyan@163.com

引用本文:

范泽文, 赵新宇, 邱帅, 王艳, 郭静, 权慧欣, 徐兰娟. 聚乳酸/聚乙二醇/羟基磷灰石多孔骨支架的3D打印制备及其生物相容性[J]. 材料工程, 2021, 49(4): 135-141.
Ze-wen FAN, Xin-yu ZHAO, Shuai QIU, Yan WANG, Jing GUO, Hui-xin QUAN, Lan-juan XU. 3D printing of polylactic acid/poly ethylene glycol/hydroxyapatite porous bone scaffolds and their biocompatibility. Journal of Materials Engineering, 2021, 49(4): 135-141.

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http://jme.biam.ac.cn/CN/10.11868/j.issn.1001-4381.2020.000390 或 http://jme.biam.ac.cn/CN/Y2021/V49/I4/135

Fig.1 3D打印拉伸、冲击试样模型(a)和多孔支架模型(b)

Fig.2 PLA/PEG/HA线材的拉伸性能曲线

Table 1 PLA/PEG/HA线材的拉伸性能

Fig.3 PLA/PEG/HA复合材料的DCS曲线

Table 2 PLA/PEG/HA复合材料的结晶参数

Fig.4 PLA/PEG/HA复合材料的表观黏度(η*)与剪切速率(ω)曲线

Fig.5 3D打印拉伸和冲击试样的光学图片

Fig.6 3D打印试样的拉伸性能曲线

Table 3 3D打印PLA/PEG/HA复合材料的冲击性能

Fig.7 PLA/PEG/HA复合材料多孔支架的SEM照片
(a)，(b)表面；(c)，(d)截面；(e)，(f)表面放大图像

Fig.8 大鼠间充质干细胞在多孔支架上的生物相容性测试
(a)，(b)细胞在生物支架表面的黏附；(c)荧光标记的细胞骨架；(d)荧光标记的细胞核

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